My research has focused on the functional proteomic characterization of the PKCe signaling system in the heart. Over the past 4 years, I have used two dimensional electrophoresis, mass spectrometry, immunoprecipitation, and a host other of classical biochemical and molecular biological approaches to characterize PKC complexes in the normal and protected heart. Furthermore, I am currently focussing my dissertation research project on the extensive functional characterization of a specific module within these complexes. In addition, I am in the process of developing novel techniques to analyze native protein complexes.
email: jzhang@mednet.ucla.edu
Jun Zhang, PhD
publications by Jun Zhang
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Mayr M, Liem D, Zhang J , Li X, Avliyakulov NK, Yang JI, Young G, Vondriska TM, Ladroue C, Madhu B, Griffiths JR, Gomes A, Xu Q, Ping P. Proteomic and metabolomic analysis of cardioprotection: Interplay between protein kinase C epsilon and delta in regulating glucose metabolism of murine hearts. J Mol Cell Cardiol. 2009;46:268 – 77.
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Zhang J , Liem DA, Mueller M, Wang Y, Zong C, Deng N, Vondriska TM, Korge P, Drews O, Maclellan WR, Honda H, Weiss JN, Apweiler R, Ping P. Altered proteome biology of cardiac mitochondria under stress conditions. J Proteome Res. 2008;7:2204 – 14.
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Zhang J , Li X, Mueller M, Wang Y, Zong C, Deng N, Vondriska TM, Liem DA, Yang JI, Korge P, Honda H, Weiss JN, Apweiler R, Ping P. Systematic characterization of the murine mitochondrial proteome using functionally validated cardiac mitochondria. Proteomics. 2008;8:1564 – 75.
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Zhang J , Liem DA, Mueller M, Wang Y, Zong C, Deng N, Vondriska TM, Korge P, Drews O, Maclellan WR, Honda H, Weiss JN, Apweiler R, Ping P. Altered proteome biology of cardiac mitochondria under stress conditions. J Proteome Res. 2008;7:2204 – 14.
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Liem DA, Zhao P, Angelis E, Chan SS, Zhang J , Wang G, Berthet C, Kaldis P, Ping P, MacLellan WR. Cyclin-dependent kinase 2 signaling regulates myocardial ischemia/reperfusion injury. J Mol Cell Cardiol. 2008;45:610 – 6.
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Liem DA, Honda HM, Zhang J , Woo D, Ping P. Past and present course of cardioprotection against ischemia-reperfusion injury. J Appl Physiol. 2007;103:2129 – 36.
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Mayr M, Zhang J , Greene AS, Gutterman D, Perloff J, Ping P. Proteomic based development of biomarkers in cardiovascular disease: mechanistic, clinical, and therapeutic insights. Mol Cell Proteomics. 2006;5:1853 – 1864.
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Gomes AV, Zong C, Edmondson RD, Li X, Stefani E, Zhang J , Jones RC, Thyparambil S, Wang GW, Qiao X, Bardag-Gorce F, Ping P. Mapping the murine cardiac 26S proteasome complexes. Circ Res. 2006;99:362 – 71.
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Zhang J , Baines CP, Zong C, Cardwell EM, Wang G, Vondriska TM, Ping P. Functional proteomic analysis of a three-tier PKCepsilon-Akt-eNOS signaling module in cardiac protection. Am J Physiol Heart Circ Physiol. 2005;288:H954-61 .
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Gomes AV, Zong C, Edmondson RD, Berhane BT, Wang GW, Le S, Young G, Zhang J , Vondriska TM, Whitelegge JP, Jones RC, Joshua IG, Thyparambil S, Pantaleon, D, Qiao J, Loo J, Ping P. The murine cardiac 26S proteasome: an organelle awaiting exploration. Ann N Y Acad Sci. 2005;1047:197 – 207.
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Zhang J , Ping P, Wang GW, Lu M, Pantaleon D, Tang XL, Bolli R, Vondriska TM. Bmx, a member of the Tec family of nonreceptor tyrosine kinases, is a novel participant in pharmacological cardioprotection. Am J Physiol Heart Circ Physiol. 2004;287:H2364-6 .
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Baines CP, Song CX, Zheng YT, Wang GW, Zhang J , Wang OL, Guo Y, Bolli R, Cardwell EM, Ping P. Protein kinase Cepsilon interacts with and inhibits the permeability transition pore in cardiac mitochondria. Circ Res. 2003;92:873 – 80.
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Zhang J , Ping P, Vondriska TM, Tang XL, Wang GW, Cardwell EM, Bolli R. Cardioprotection involves activation of NF-kappa B via PKC-dependent tyrosine and serine phosphorylation of I kappa B-alpha. Am J Physiol Heart Circ Physiol. 2003;285:H1753-8 .
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Pass JM, Zhang J , Vondriska TM, Ping P. Functional proteomic analysis of the protein kinase C signaling system. Methods Mol Biol. 2003;233:369 – 85.
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Ping P, Baines CP, Gu Y, Prabhu SD, Zhang J , Tsai LL, Cardwell E, Zong NC, Vondriska TM, Korge P, Bhatnagar A, Wang GW. Cardiac toxic effects of trans-2-hexenal are mediated by induction of cardiomyocyte apoptotic pathways. Cardiovasc Toxicol. 2003;3:341 – 51.
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Song C, Vondriska TM, Wang GW, Klein JB, Cao X, Zhang J , Kang YJ, D’Souza S, Ping P. Molecular conformation dictates signaling module formation: example of PKCe and Src tyrosine kinase. Am J Physiol Heart Circ Physiol. 2002;282:H1166-71 .
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Balafanova Z, Bolli R, Zhang J , Zheng Y, Pass JM, Bhatnagar A, Tang XL, Wang O, Cardwell E, Ping P. Nitric oxide (NO) induces nitration of protein kinase C epsilon (PKCe), facilitating PKCe enhanced PKCe-RACK2 interactions: a novel mechanism of no-triggered activation of PKCe. J Biol Chem. 2002;277:15021 – 15027.
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Ping P, Song C, Zhang J , Guo Y, Cao X, Li RC, Wu W, Vondriska TM, Pass JM, Tang XL, Pierce WM, Bolli R. Formation of protein kinase Ce-Lck signaling modules confers cardioprotection. J Clin Invest. 2002;109:499 – 507.
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Baines CP, Zhang J , Wang GW, Zheng YT, Xiu JX, Cardwell EM, Bolli R, Ping P. Mitochondrial PKCe and MAPK form signaling modules in the murine heart: enhanced mitochondrial PKCe-MAPK interactions and differential MAPK activation in PKCe-induced cardioprotection. Circ Res. 2002;90:390 – 7.
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Edmondson RD, Vondriska TM, Biederman KJ, Zhang J , Jones RC, Zheng Y, Allen DL, Xiu JX, Cardwell EM, Pisano MR, Ping P. Protein kinase C e signaling complexes include metabolism– and transcription/translation-related proteins: complimentary separation techniques with LC/MS/MS. Mol Cell Proteomics. 2002;1:421 – 33.
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Ping P, Zhang J , Pierce WM Jr, Bolli R. Functional proteomic analysis of protein kinase C epsilon signaling complexes in the normal heart and during cardioprotection. Circ Res. 2001;88:59 – 62.
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Pass JM, Zheng Y, Wead WB, Zhang J , Li RC, Bolli R, Ping P. PKCepsilon activation induces dichotomous cardiac phenotypes and modulates PKCepsilon-RACK interactions and RACK expression. Am J Physiol Heart Circ Physiol. 2001;280:H946-55 .
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Vondriska TM, Zhang J , Song C, Tang XL, Cao X, Baines CP, Pass JM, Wang S, Bolli R, Ping P. Protein kinase C epsilon-Src modules direct signal transduction in nitric oxide-induced cardioprotection: complex formation as a means for cardioprotective signaling . Circ Res . 2001;88:1306 – 13.
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Pass JM, Gao J, Jones WK, Wead WB, Wu X, Zhang J , Baines CP, Bolli R, Zheng YT, Joshua IG, Ping P. Enhanced PKC beta II translocation and PKC beta II-RACK1 interactions in PKC epsilon-induced heart failure: a role for RACK1 . Am J Physiol Heart Circ Physiol . 2001;281:H2500-10 .
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Li RC, Ping P, Zhang J , Wead WB, Cao X, Gao J, Zheng Y, Huang S, Han J, Bolli R. PKCepsilon modulates NF-kappaB and AP-1 via mitogen-activated protein kinases in adult rabbit cardiomyocytes . Am J Physiol Heart Circ Physiol . 2000;279:H1679-89 .
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Ping P, Takano H, Zhang J , Tang XL, Qiu Y, Li RC, Banerjee S, Dawn B, Balafanova Z, Bolli R. Isoform-selective activation of protein kinase C by nitric oxide in the heart of conscious rabbits: a signaling mechanism for both nitric oxide-induced and ischemia-induced preconditioning . Circ Res . 1999;84:587 – 604.
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Ping P, Zhang J , Cao X, Li RC, Kong D, Tang XL, Qiu Y, Manchikalapudi S, Auchampach JA, Black RG, Bolli R. PKC-dependent activation of p44/p42 MAPKs during myocardial ischemia-reperfusion in conscious rabbits . Am J Physiol . 1999;276:H1468-81 .
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Ping P, Zhang J , Zheng YT, Li RC, Dawn B, Tang XL, Takano H, Balafanova Z, Bolli R. Demonstration of selective protein kinase C-dependent activation of Src and Lck tyrosine kinases during ischemic preconditioning in conscious rabbits . Circ Res . 1999;85:542 – 50.
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Ping P, Zhang J , Huang S, Cao X, Tang XL, Li RC, Zheng YT, Qiu Y, Clerk A, Sugden P, Han J, Bolli R. PKC-dependent activation of p46/p54 JNKs during ischemic preconditioning in conscious rabbits . Am J Physiol . 1999;277:H1771-85 .
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Qiu Y, Ping P, Tang XL, Manchikalapudi S, Rizvi A, Zhang J , Takano H, Wu WJ, Teschner S, Bolli R. Direct evidence that protein kinase C plays an essential role in the development of late preconditioning against myocardial stunning in conscious rabbits and that epsilon is the isoform involved . J Clin Invest . 1998;101:2182 – 98.
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Bolli R, Dawn B, Tang XL, Qiu Y, Ping P, Xuan YT, Jones WK, Takano H, Guo Y, Zhang J . The nitric oxide hypothesis of late preconditioning . Basic Res Cardiol . 1998;93:325 – 38.
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Ping P, Zhang J , Qiu Y, Tang XL, Manchikalapudi S, Cao X, Bolli R. Ischemic preconditioning induces selective translocation of protein kinase C isoforms epsilon and eta in the heart of conscious rabbits without subcellular redistribution of total protein kinase C activity . Circ Res . 1997;81:404 – 14.
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Zhang J , Xu JL. Oleyl oleate synthesis by immobilized lipase from Candida sp-1619 . Chinese J of Biotechnology. 1995;11:243 – 252.
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Xu JL, Zhang J . Studies on the lipase from Penicillium Camembertii-PG3 . Acta Mycologica Sinica. 1995;14:136 – 142.