War­ning! War­ning!! Pro­tein puri­fi­ca­tion can be hazar­dous. Sean deve­lo­ped pneu­mo­nia after spen­ding few hours in cold room. Cool guys and hot girls, whoe­ver you are, please take cau­tion and main­tain your core tem­pe­ra­ture. If you do not have a ther­mo­me­ter of your own, you can always use David’s.